Journal: The Journal of Biological Chemistry

Article Title: Homeostatic Competition between Phasic and Tonic Inhibition *

doi: 10.1074/jbc.M113.491464

Figure Lengend Snippet: Ectopic expression of α6 subunit-induced δ subunit expression in pyramidal neurons. A and B, a control neuron transfected with mCherry (red) showed a very low level of δ immunofluorescence signal (green). C and D, two representative neurons transfected with the α6 subunit and mCherry showed increased an δ immunofluorescence signal on the soma and dendrites. Scale bars = 20 μm. E, quantified fluorescence intensity showing elevated δ expression in α6-transfected neurons (n = 24 in both groups). ***, p < 0.001, unpaired Student's t test. a.u., arbitrary units.

Article Snippet: Cultured neurons were transfected with mCherry alone or α6 plus mCherry, and the antibodies specific for the δ subunit (PhosphoSolutions) were used to label the neuronal surface expression in the non-permeabilized condition.

Techniques: Expressing, Control, Transfection, Immunofluorescence, Fluorescence

Journal: The Journal of Biological Chemistry

Article Title: Homeostatic Competition between Phasic and Tonic Inhibition *

doi: 10.1074/jbc.M113.491464

Figure Lengend Snippet: Overexpression of α5-GABAA receptors also decreased synaptic GABAergic transmission. A–C, control hippocampal neurons overexpressing mCherry showed no HA staining. D–F, hippocampal neurons overexpressing the HA-α5β3γ2 subunits showed a substantial HA staining signal throughout the soma and dendrites. G and H, representative traces showing reduced mIPSC events in hippocampal neurons overexpressing α5-GABAA receptors. Non-TF, non-transfected. I and J, quantitative data showing the reduction of mIPSC frequency but not the amplitude after overexpressing α5-GABAA receptors (n = 10–11). ***, p < 0.001, Student's t test. Scale bars = 20 μm for A, B, D, and E and 5 μm for C and F.

Article Snippet: Cultured neurons were transfected with mCherry alone or α6 plus mCherry, and the antibodies specific for the δ subunit (PhosphoSolutions) were used to label the neuronal surface expression in the non-permeabilized condition.

Techniques: Over Expression, Transmission Assay, Control, Staining, Transfection